Variable Oligomerization Modes in Coronavirus Non-structural Protein 9
Identifieur interne : 000777 ( new/Analysis ); précédent : 000776; suivant : 000778Variable Oligomerization Modes in Coronavirus Non-structural Protein 9
Auteurs : Rajesh Ponnusamy [Allemagne] ; Ralf Moll [Allemagne] ; Thomas Weimar [Allemagne] ; Jeroen R. Mesters [Allemagne] ; Rolf Hilgenfeld [Allemagne]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 2008.
Abstract
Non-structural protein 9 (Nsp9) of coronaviruses is believed to bind single-stranded RNA in the viral replication complex. The crystal structure of Nsp9 of human coronavirus (HCoV) 229E reveals a novel disulfide-linked homodimer, which is very different from the previously reported Nsp9 dimer of SARS coronavirus. In contrast, the structure of the Cys69Ala mutant of HCoV-229E Nsp9 shows the same dimer organization as the SARS-CoV protein. In the crystal, the wild-type HCoV-229E protein forms a trimer of dimers, whereas the mutant and SARS-CoV Nsp9 are organized in rod-like polymers. Chemical cross-linking suggests similar modes of aggregation in solution. In zone-interference gel electrophoresis assays and surface plasmon resonance experiments, the HCoV-229E wild-type protein is found to bind oligonucleotides with relatively high affinity, whereas binding by the Cys69Ala and Cys69Ser mutants is observed only for the longest oligonucleotides. The corresponding mutations in SARS-CoV Nsp9 do not hamper nucleic acid binding. From the crystal structures, a model for single-stranded RNA binding by Nsp9 is deduced. We propose that both forms of the Nsp9 dimer are biologically relevant; the occurrence of the disulfide-bonded form may be correlated with oxidative stress induced in the host cell by the viral infection.
Url:
DOI: 10.1016/j.jmb.2008.07.071
PubMed: 18694760
PubMed Central: 7094590
Affiliations:
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Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Moll, Ralf" sort="Moll, Ralf" uniqKey="Moll R" first="Ralf" last="Moll">Ralf Moll</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck</wicri:regionArea><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Weimar, Thomas" sort="Weimar, Thomas" uniqKey="Weimar T" first="Thomas" last="Weimar">Thomas Weimar</name><affiliation wicri:level="1"><nlm:aff id="aff2">Institute of Chemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Chemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck</wicri:regionArea><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Mesters, Jeroen R" sort="Mesters, Jeroen R" uniqKey="Mesters J" first="Jeroen R." last="Mesters">Jeroen R. Mesters</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck</wicri:regionArea><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion></affiliation></author><author><name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name><affiliation wicri:level="1"><nlm:aff id="aff1">Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck</wicri:regionArea><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion><wicri:noRegion>23538 Lübeck</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, Building 22a, Notkestr. 85, 22603 Hamburg, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Laboratory for Structural Biology of Infection and Inflammation, c/o DESY, Building 22a, Notkestr. 85, 22603 Hamburg</wicri:regionArea><wicri:noRegion>22603 Hamburg</wicri:noRegion><placeName><settlement type="city">Hambourg</settlement><region type="land" nuts="2">Hambourg</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Molecular Biology</title><idno type="ISSN">0022-2836</idno><idno type="eISSN">1089-8638</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Non-structural protein 9 (Nsp9) of coronaviruses is believed to bind single-stranded RNA in the viral replication complex. The crystal structure of Nsp9 of human coronavirus (HCoV) 229E reveals a novel disulfide-linked homodimer, which is very different from the previously reported Nsp9 dimer of SARS coronavirus. In contrast, the structure of the Cys69Ala mutant of HCoV-229E Nsp9 shows the same dimer organization as the SARS-CoV protein. In the crystal, the wild-type HCoV-229E protein forms a trimer of dimers, whereas the mutant and SARS-CoV Nsp9 are organized in rod-like polymers. Chemical cross-linking suggests similar modes of aggregation in solution. In zone-interference gel electrophoresis assays and surface plasmon resonance experiments, the HCoV-229E wild-type protein is found to bind oligonucleotides with relatively high affinity, whereas binding by the Cys69Ala and Cys69Ser mutants is observed only for the longest oligonucleotides. The corresponding mutations in SARS-CoV Nsp9 do not hamper nucleic acid binding. From the crystal structures, a model for single-stranded RNA binding by Nsp9 is deduced. We propose that both forms of the Nsp9 dimer are biologically relevant; the occurrence of the disulfide-bonded form may be correlated with oxidative stress induced in the host cell by the viral infection.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S. van der Werf</name></author><author><name sortKey="Brodt, H R" uniqKey="Brodt H">H.R. Brodt</name></author><author><name sortKey="Becker, S" uniqKey="Becker S">S. Becker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D. Erdman</name></author><author><name sortKey="Goldsmith, C S" uniqKey="Goldsmith C">C.S. Goldsmith</name></author><author><name sortKey="Zaki, S R" uniqKey="Zaki S">S.R. Zaki</name></author><author><name sortKey="Peret, T" uniqKey="Peret T">T. Peret</name></author><author><name sortKey="Emery, S" uniqKey="Emery S">S. Emery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T. Kuiken</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author><author><name sortKey="Schutten, M" uniqKey="Schutten M">M. Schutten</name></author><author><name sortKey="Rimmelzwaan, G F" uniqKey="Rimmelzwaan G">G.F. Rimmelzwaan</name></author><author><name sortKey="Van Amerongen, G" uniqKey="Van Amerongen G">G. van Amerongen</name></author><author><name sortKey="Van Riel, D" uniqKey="Van Riel D">D. van Riel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J.S. Peiris</name></author><author><name sortKey="Chu, C M" uniqKey="Chu C">C.M. Chu</name></author><author><name sortKey="Cheng, V C" uniqKey="Cheng V">V.C. Cheng</name></author><author><name sortKey="Chan, K S" uniqKey="Chan K">K.S. Chan</name></author><author><name sortKey="Hung, I F" uniqKey="Hung I">I.F. Hung</name></author><author><name sortKey="Poon, L L" uniqKey="Poon L">L.L. Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Groneberg, D A" uniqKey="Groneberg D">D.A. Groneberg</name></author><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R. Hilgenfeld</name></author><author><name sortKey="Zabel, P" uniqKey="Zabel P">P. Zabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L. van der Hoek</name></author><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K. Pyrc</name></author><author><name sortKey="Jebbink, M F" uniqKey="Jebbink M">M.F. Jebbink</name></author><author><name sortKey="Vermeulen Oost, W" uniqKey="Vermeulen Oost W">W. Vermeulen-Oost</name></author><author><name sortKey="Berkhout, R J" uniqKey="Berkhout R">R.J. Berkhout</name></author><author><name sortKey="Wolthers, K C" uniqKey="Wolthers K">K.C. Wolthers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, P C" uniqKey="Woo P">P.C. Woo</name></author><author><name sortKey="Lau, S K" uniqKey="Lau S">S.K. Lau</name></author><author><name sortKey="Chu, C M" uniqKey="Chu C">C.M. Chu</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K.H. Chan</name></author><author><name sortKey="Tsoi, H W" uniqKey="Tsoi H">H.W. Tsoi</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y. Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name></author><author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name></author><author><name sortKey="Spaan, W J" uniqKey="Spaan W">W.J. Spaan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D. Cavanagh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lai, M M" uniqKey="Lai M">M.M. Lai</name></author><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D. Cavanagh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Herold, J" uniqKey="Herold J">J. Herold</name></author><author><name sortKey="Raabe, T" uniqKey="Raabe T">T. Raabe</name></author><author><name sortKey="Schelle Prinz, B" uniqKey="Schelle Prinz B">B. Schelle-Prinz</name></author><author><name sortKey="Siddell, S G" uniqKey="Siddell S">S.G. Siddell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Herold, J" uniqKey="Herold J">J. Herold</name></author><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name></author><author><name sortKey="Thiel, V" uniqKey="Thiel V">V. Thiel</name></author><author><name sortKey="Schelle, B" uniqKey="Schelle B">B. Schelle</name></author><author><name sortKey="Siddell, S G" uniqKey="Siddell S">S.G. Siddell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J. Ziebuhr</name></author><author><name sortKey="Siddell, S G" uniqKey="Siddell S">S.G. Siddell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anand, K" uniqKey="Anand K">K. Anand</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J. Ziebuhr</name></author><author><name sortKey="Wadhwani, P" uniqKey="Wadhwani P">P. Wadhwani</name></author><author><name sortKey="Mesters, J R" uniqKey="Mesters J">J.R. Mesters</name></author><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R. Hilgenfeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anand, K" uniqKey="Anand K">K. Anand</name></author><author><name sortKey="Palm, G J" uniqKey="Palm G">G.J. Palm</name></author><author><name sortKey="Mesters, J R" uniqKey="Mesters J">J.R. Mesters</name></author><author><name sortKey="Siddell, S G" uniqKey="Siddell S">S.G. Siddell</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J. Ziebuhr</name></author><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R. Hilgenfeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, H" uniqKey="Yang H">H. Yang</name></author><author><name sortKey="Yang, M" uniqKey="Yang M">M. Yang</name></author><author><name sortKey="Ding, Y" uniqKey="Ding Y">Y. Ding</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Lou, Z" uniqKey="Lou Z">Z. Lou</name></author><author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z. Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, J" uniqKey="Tan J">J. Tan</name></author><author><name sortKey="Verschueren, K H" uniqKey="Verschueren K">K.H. Verschueren</name></author><author><name sortKey="Anand, K" uniqKey="Anand K">K. Anand</name></author><author><name sortKey="Shen, J" uniqKey="Shen J">J. Shen</name></author><author><name sortKey="Yang, M" uniqKey="Yang M">M. Yang</name></author><author><name sortKey="Xu, Y" uniqKey="Xu Y">Y. Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matthes, N" uniqKey="Matthes N">N. Matthes</name></author><author><name sortKey="Mesters, J R" uniqKey="Mesters J">J.R. Mesters</name></author><author><name sortKey="Coutard, B" uniqKey="Coutard B">B. Coutard</name></author><author><name sortKey="Canard, B" uniqKey="Canard B">B. Canard</name></author><author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name></author><author><name sortKey="Moll, R" uniqKey="Moll R">R. Moll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ponnusamy, R" uniqKey="Ponnusamy R">R. Ponnusamy</name></author><author><name sortKey="Mesters, J R" uniqKey="Mesters J">J.R. Mesters</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J. Ziebuhr</name></author><author><name sortKey="Moll, R" uniqKey="Moll R">R. Moll</name></author><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R. Hilgenfeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Robertson, M P" uniqKey="Robertson M">M.P. Robertson</name></author><author><name sortKey="Igel, H" uniqKey="Igel H">H. Igel</name></author><author><name sortKey="Baertsch, R" uniqKey="Baertsch R">R. Baertsch</name></author><author><name sortKey="Haussler, D" uniqKey="Haussler D">D. Haussler</name></author><author><name sortKey="Ares, M" uniqKey="Ares M">M. Ares</name></author><author><name sortKey="Scott, W G" uniqKey="Scott W">W.G. Scott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imbert, I" uniqKey="Imbert I">I. Imbert</name></author><author><name sortKey="Guillemot, J C" uniqKey="Guillemot J">J.C. Guillemot</name></author><author><name sortKey="Bourhis, J M" uniqKey="Bourhis J">J.M. Bourhis</name></author><author><name sortKey="Bussetta, C" uniqKey="Bussetta C">C. Bussetta</name></author><author><name sortKey="Coutard, B" uniqKey="Coutard B">B. Coutard</name></author><author><name sortKey="Egloff, M P" uniqKey="Egloff M">M.P. Egloff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhai, Y" uniqKey="Zhai Y">Y. Zhai</name></author><author><name sortKey="Sun, F" uniqKey="Sun F">F. Sun</name></author><author><name sortKey="Li, X" uniqKey="Li X">X. Li</name></author><author><name sortKey="Pang, H" uniqKey="Pang H">H. Pang</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X. Xu</name></author><author><name sortKey="Bartlam, M" uniqKey="Bartlam M">M. Bartlam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sutton, G" uniqKey="Sutton G">G. Sutton</name></author><author><name sortKey="Fry, E" uniqKey="Fry E">E. Fry</name></author><author><name sortKey="Carter, L" uniqKey="Carter L">L. Carter</name></author><author><name sortKey="Sainsbury, S" uniqKey="Sainsbury S">S. Sainsbury</name></author><author><name sortKey="Walter, T" uniqKey="Walter T">T. Walter</name></author><author><name sortKey="Nettleship, J" uniqKey="Nettleship J">J. Nettleship</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bost, A G" uniqKey="Bost A">A.G. Bost</name></author><author><name sortKey="Carnahan, R H" uniqKey="Carnahan R">R.H. Carnahan</name></author><author><name sortKey="Lu, X T" uniqKey="Lu X">X.T. Lu</name></author><author><name sortKey="Denison, M R" uniqKey="Denison M">M.R. Denison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Egloff, M P" uniqKey="Egloff M">M.P. Egloff</name></author><author><name sortKey="Ferron, F" uniqKey="Ferron F">F. Ferron</name></author><author><name sortKey="Campanacci, V" uniqKey="Campanacci V">V. Campanacci</name></author><author><name sortKey="Longhi, S" uniqKey="Longhi S">S. Longhi</name></author><author><name sortKey="Rancurel, C" uniqKey="Rancurel C">C. Rancurel</name></author><author><name sortKey="Dutartre, H" uniqKey="Dutartre H">H. Dutartre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laskowski, R A" uniqKey="Laskowski R">R.A. Laskowski</name></author><author><name sortKey="Macarthur, M W" uniqKey="Macarthur M">M.W. MacArthur</name></author><author><name sortKey="Moss, D S" uniqKey="Moss D">D.S. Moss</name></author><author><name sortKey="Thornton, J M" uniqKey="Thornton J">J.M. Thornton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Theobald, D L" uniqKey="Theobald D">D.L. Theobald</name></author><author><name sortKey="Mitton Fry, R M" uniqKey="Mitton Fry R">R.M. Mitton-Fry</name></author><author><name sortKey="Wuttke, D S" uniqKey="Wuttke D">D.S. Wuttke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Webster, G" uniqKey="Webster G">G. Webster</name></author><author><name sortKey="Genschel, J" uniqKey="Genschel J">J. Genschel</name></author><author><name sortKey="Curth, U" uniqKey="Curth U">U. Curth</name></author><author><name sortKey="Urbanke, C" uniqKey="Urbanke C">C. Urbanke</name></author><author><name sortKey="Kang, C" uniqKey="Kang C">C. Kang</name></author><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R. Hilgenfeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bochkarev, A" uniqKey="Bochkarev A">A. Bochkarev</name></author><author><name sortKey="Pfuetzner, R A" uniqKey="Pfuetzner R">R.A. Pfuetzner</name></author><author><name sortKey="Edwards, A M" uniqKey="Edwards A">A.M. Edwards</name></author><author><name sortKey="Frappier, L" uniqKey="Frappier L">L. Frappier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mapelli, M" uniqKey="Mapelli M">M. Mapelli</name></author><author><name sortKey="Panjikar, S" uniqKey="Panjikar S">S. Panjikar</name></author><author><name sortKey="Tucker, P A" uniqKey="Tucker P">P.A. Tucker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anand, K" uniqKey="Anand K">K. Anand</name></author><author><name sortKey="Pal, D" uniqKey="Pal D">D. Pal</name></author><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R. Hilgenfeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, B" uniqKey="Lee B">B. Lee</name></author><author><name sortKey="Richards, F M" uniqKey="Richards F">F.M. Richards</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ellman, G L" uniqKey="Ellman G">G.L. Ellman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abrahams, J P" uniqKey="Abrahams J">J.P. Abrahams</name></author><author><name sortKey="Kraal, B" uniqKey="Kraal B">B. Kraal</name></author><author><name sortKey="Bosch, L" uniqKey="Bosch L">L. Bosch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bessette, P H" uniqKey="Bessette P">P.H. Bessette</name></author><author><name sortKey="Aslund, F" uniqKey="Aslund F">F. Aslund</name></author><author><name sortKey="Beckwith, J" uniqKey="Beckwith J">J. Beckwith</name></author><author><name sortKey="Georgiou, G" uniqKey="Georgiou G">G. Georgiou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parks, D" uniqKey="Parks D">D. Parks</name></author><author><name sortKey="Bolinger, R" uniqKey="Bolinger R">R. Bolinger</name></author><author><name sortKey="Mann, K" uniqKey="Mann K">K. Mann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lawrence, M C" uniqKey="Lawrence M">M.C. Lawrence</name></author><author><name sortKey="Colman, P M" uniqKey="Colman P">P.M. Colman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chothia, C" uniqKey="Chothia C">C. Chothia</name></author><author><name sortKey="Levitt, M" uniqKey="Levitt M">M. Levitt</name></author><author><name sortKey="Richardson, D" uniqKey="Richardson D">D. Richardson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Russ, W P" uniqKey="Russ W">W.P. Russ</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D.M. Engelman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Senes, A" uniqKey="Senes A">A. Senes</name></author><author><name sortKey="Gerstein, M" uniqKey="Gerstein M">M. Gerstein</name></author><author><name sortKey="Engelman, D M" uniqKey="Engelman D">D.M. Engelman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, C Y" uniqKey="Chen C">C.Y. Chen</name></author><author><name sortKey="Chang, C K" uniqKey="Chang C">C.K. Chang</name></author><author><name sortKey="Chang, Y W" uniqKey="Chang Y">Y.W. Chang</name></author><author><name sortKey="Sue, S C" uniqKey="Sue S">S.C. Sue</name></author><author><name sortKey="Bai, H I" uniqKey="Bai H">H.I. Bai</name></author><author><name sortKey="Riang, L" uniqKey="Riang L">L. Riang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petersen, M T" uniqKey="Petersen M">M.T. Petersen</name></author><author><name sortKey="Jonson, P H" uniqKey="Jonson P">P.H. Jonson</name></author><author><name sortKey="Petersen, S B" uniqKey="Petersen S">S.B. Petersen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stevens, R" uniqKey="Stevens R">R. Stevens</name></author><author><name sortKey="Stevens, L" uniqKey="Stevens L">L. Stevens</name></author><author><name sortKey="Price, N C" uniqKey="Price N">N.C. Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="You, J S" uniqKey="You J">J.S. You</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M. Wang</name></author><author><name sortKey="Lee, S H" uniqKey="Lee S">S.H. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bauer, C E" uniqKey="Bauer C">C.E. Bauer</name></author><author><name sortKey="Elsen, S" uniqKey="Elsen S">S. Elsen</name></author><author><name sortKey="Bird, T H" uniqKey="Bird T">T.H. Bird</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eleouet, J F" uniqKey="Eleouet J">J.F. Eleouet</name></author><author><name sortKey="Chilmonczyk, S" uniqKey="Chilmonczyk S">S. Chilmonczyk</name></author><author><name sortKey="Besnardeau, L" uniqKey="Besnardeau L">L. Besnardeau</name></author><author><name sortKey="Laude, H" uniqKey="Laude H">H. Laude</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaul, P" uniqKey="Kaul P">P. Kaul</name></author><author><name sortKey="Biagioli, M C" uniqKey="Biagioli M">M.C. Biagioli</name></author><author><name sortKey="Singh, I" uniqKey="Singh I">I. Singh</name></author><author><name sortKey="Turner, R B" uniqKey="Turner R">R.B. Turner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Oberley, L W" uniqKey="Oberley L">L.W. Oberley</name></author><author><name sortKey="Murhammer, D W" uniqKey="Murhammer D">D.W. Murhammer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mikhailov, V S" uniqKey="Mikhailov V">V.S. Mikhailov</name></author><author><name sortKey="Okano, K" uniqKey="Okano K">K. Okano</name></author><author><name sortKey="Rohrmann, G F" uniqKey="Rohrmann G">G.F. Rohrmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mcbride, A A" uniqKey="Mcbride A">A.A. McBride</name></author><author><name sortKey="Klausner, R D" uniqKey="Klausner R">R.D. Klausner</name></author><author><name sortKey="Howley, P M" uniqKey="Howley P">P.M. Howley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sampson, D A" uniqKey="Sampson D">D.A. Sampson</name></author><author><name sortKey="Arana, M E" uniqKey="Arana M">M.E. Arana</name></author><author><name sortKey="Boehmer, P E" uniqKey="Boehmer P">P.E. Boehmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knipe, D M" uniqKey="Knipe D">D.M. Knipe</name></author><author><name sortKey="Quinlan, M P" uniqKey="Quinlan M">M.P. Quinlan</name></author><author><name sortKey="Spang, A E" uniqKey="Spang A">A.E. Spang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dudas, K C" uniqKey="Dudas K">K.C. Dudas</name></author><author><name sortKey="Ruyechan, W T" uniqKey="Ruyechan W">W.T. Ruyechan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gosert, R" uniqKey="Gosert R">R. Gosert</name></author><author><name sortKey="Kanjanahaluethai, A" uniqKey="Kanjanahaluethai A">A. Kanjanahaluethai</name></author><author><name sortKey="Egger, D" uniqKey="Egger D">D. Egger</name></author><author><name sortKey="Bienz, K" uniqKey="Bienz K">K. Bienz</name></author><author><name sortKey="Baker, S C" uniqKey="Baker S">S.C. Baker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Prentice, E" uniqKey="Prentice E">E. Prentice</name></author><author><name sortKey="Jerome, W G" uniqKey="Jerome W">W.G. Jerome</name></author><author><name sortKey="Yoshimori, T" uniqKey="Yoshimori T">T. Yoshimori</name></author><author><name sortKey="Mizushima, N" uniqKey="Mizushima N">N. Mizushima</name></author><author><name sortKey="Denison, M R" uniqKey="Denison M">M.R. Denison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name></author><author><name sortKey="Van Der Meer, Y" uniqKey="Van Der Meer Y">Y. van der Meer</name></author><author><name sortKey="Zevenhoven Dobbe, J" uniqKey="Zevenhoven Dobbe J">J. Zevenhoven-Dobbe</name></author><author><name sortKey="Onderwater, J J" uniqKey="Onderwater J">J.J. Onderwater</name></author><author><name sortKey="Van Der Meulen, J" uniqKey="Van Der Meulen J">J. van der Meulen</name></author><author><name sortKey="Koerten, H K" uniqKey="Koerten H">H.K. Koerten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Hemert, M J" uniqKey="Van Hemert M">M.J. van Hemert</name></author><author><name sortKey="Van Den Worm, S H E" uniqKey="Van Den Worm S">S.H.E. van den Worm</name></author><author><name sortKey="Knoops, K" uniqKey="Knoops K">K. Knoops</name></author><author><name sortKey="Mommaas, A M" uniqKey="Mommaas A">A.M. Mommaas</name></author><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name></author><author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, Y H" uniqKey="Wu Y">Y.H. Wu</name></author><author><name sortKey="Tseng, C P" uniqKey="Tseng C">C.P. Tseng</name></author><author><name sortKey="Cheng, M L" uniqKey="Cheng M">M.L. Cheng</name></author><author><name sortKey="Ho, H Y" uniqKey="Ho H">H.Y. Ho</name></author><author><name sortKey="Shih, S R" uniqKey="Shih S">S.R. Shih</name></author><author><name sortKey="Chiu, D T" uniqKey="Chiu D">D.T. Chiu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imai, Y" uniqKey="Imai Y">Y. Imai</name></author><author><name sortKey="Kuba, K" uniqKey="Kuba K">K. Kuba</name></author><author><name sortKey="Neely, G G" uniqKey="Neely G">G.G. Neely</name></author><author><name sortKey="Yaghubian Malhami, R" uniqKey="Yaghubian Malhami R">R. Yaghubian-Malhami</name></author><author><name sortKey="Perkmann, T" uniqKey="Perkmann T">T. Perkmann</name></author><author><name sortKey="Van Loo, G" uniqKey="Van Loo G">G. van Loo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miseta, A" uniqKey="Miseta A">A. Miseta</name></author><author><name sortKey="Csutora, P" uniqKey="Csutora P">P. Csutora</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lindwall, G" uniqKey="Lindwall G">G. Lindwall</name></author><author><name sortKey="Chau, M" uniqKey="Chau M">M. Chau</name></author><author><name sortKey="Gardner, S R" uniqKey="Gardner S">S.R. Gardner</name></author><author><name sortKey="Kohlstaedt, L A" uniqKey="Kohlstaedt L">L.A. Kohlstaedt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Otwinowski, Z" uniqKey="Otwinowski Z">Z. Otwinowski</name></author><author><name sortKey="Minor, W" uniqKey="Minor W">W. Minor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collaborative Computational Project Number" uniqKey="Collaborative Computational Project Number">Collaborative Computational Project Number 4</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mccoy, A J" uniqKey="Mccoy A">A.J. McCoy</name></author><author><name sortKey="Grosse Kunstleve, R W" uniqKey="Grosse Kunstleve R">R.W. Grosse-Kunstleve</name></author><author><name sortKey="Storoni, L C" uniqKey="Storoni L">L.C. Storoni</name></author><author><name sortKey="Read, R J" uniqKey="Read R">R.J. Read</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Emsley, P" uniqKey="Emsley P">P. Emsley</name></author><author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K. Cowtan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murshudov, G N" uniqKey="Murshudov G">G.N. Murshudov</name></author><author><name sortKey="Vagin, A A" uniqKey="Vagin A">A.A. Vagin</name></author><author><name sortKey="Dodson, E J" uniqKey="Dodson E">E.J. Dodson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cohen, G E" uniqKey="Cohen G">G.E. Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Delano, W L" uniqKey="Delano W">W.L. DeLano</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Ponnusamy, Rajesh" sort="Ponnusamy, Rajesh" uniqKey="Ponnusamy R" first="Rajesh" last="Ponnusamy">Rajesh Ponnusamy</name></noRegion><name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name><name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name><name sortKey="Mesters, Jeroen R" sort="Mesters, Jeroen R" uniqKey="Mesters J" first="Jeroen R." last="Mesters">Jeroen R. Mesters</name><name sortKey="Moll, Ralf" sort="Moll, Ralf" uniqKey="Moll R" first="Ralf" last="Moll">Ralf Moll</name><name sortKey="Weimar, Thomas" sort="Weimar, Thomas" uniqKey="Weimar T" first="Thomas" last="Weimar">Thomas Weimar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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